منابع مشابه
Purification and partial characterization of testicular hyaluronidase.
A new method is presented for the purification of testicular hyaluronidase involving ion exchange chromatography followed by gel filtration on Sephadex G-75. A highly purified hyaluronidase preparation has been obtained which contains 45,000 National Formulary activity units per mg, dry weight, and which migrates as a single component on polyacrylamide gel electrophoresis at pH 4.3. The enzyme ...
متن کاملHyaluronidase in ocular tissue. I. Sensitive biological assay for small concentrations of hyaluronidase.
(1) The spreading reaction is a biological method, measuring the decomposing effect of hyaluronidase on the mucoids of the cement substance of rabbit's skin (Duran-Reynals, 1929). These substances consist partly of hyaluronic acid. Hyaluronidase increases the permeability of the tissues, and this involves an increased spreading capacity of liquids and corpuscular substances. This test is not sp...
متن کاملStructure of dermatan sulfate. I. Degradation by testicular hyaluronidase.
The presence of D-glucuronic acid in dermatan sulfate preparations has previously been reported from several laboratories. It has not been clear whether the D-glucuronic acid represents a contamination with another polymer or whether it is actually part of the dermatan sulfate molecule. The present studies were undertaken in order to evaluate this problem more definitively. It is demonstrated t...
متن کاملThe Purification of Hypertensin I
The purification of hypertensin I has been described. The final product which is four times as powerful a pressor agent as l-arterenol, is obtained with an over-all recovery of 40 per cent. The product consists of a single component in countercurrent distribution, having a nitrogen content of 15.97 per cent and a specific activity of 7050 Goldblatt units per mg. of N or 1125 units per mg. of so...
متن کاملHeparin-sepharose affinity chromatography for purification of bull seminal-plasma hyaluronidase.
Bull seminal-plasma hyaluronidase was purified 180-fold by chromatography on concanvalin A-Sepharose, heparin Sepharose, Sephadex G-200 and Sephacryl S-200. With hyaluronic acid as the substrate, the specific activity and turnover number of purified hyaluronidase were 3.63 mumol/min per mg (104000 National Formulary units/mg of protein) and 214 min-1 (mol of product formed/mol of enzyme per min...
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ژورنال
عنوان ژورنال: YAKUGAKU ZASSHI
سال: 1960
ISSN: 0031-6903,1347-5231
DOI: 10.1248/yakushi1947.80.12_1765